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KCL-22

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Cat.No.
CSC-C0528
Description
Established from the pleural effusion of a 32-year-old woman with Philadelphia chromosome-positive CML in blast crisis in 1981; described to contain the t(9;22) leading to BCR-ABL1 e13-a2 (b2-a2) fusion gene and a p53 mutation
Organism
Human
Karyotype
Human hyperdiploid karyotype with 3.3% polyploidy - 51(47-51)<2n>X, -X, +1, +6, +8, +8, +14, +22, del(1)(p22), t(9;22)(q34;q11), add(17)(p12-13), i(21q), der(22)t(9;22)(q34;q11) - carries Phx2 - matches published karyotype
Source
chronic myeloid leukemia in blast crisis
Culture Properties
suspension
Morphology
round, single cells in suspension, sometimes in small clusters
Recommended Medium
Quality Control
Mycoplasma: contamination was eliminated with Mycoplasma Removal Agent (MRA), then negative in microbiological culture, RNA hybridization, PCR assays
Immunology: CD3 -, CD4 +, CD13 +, CD14 (+), CD15 +, CD19 -, CD25 -, HLA-DR -
Viruses: PCR: EBV -, HBV -,
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 4.5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
CSC-6249W
CSC-6250W
CSC-6280W
CSC-6281W
CSC-6282W
CSC-6296W
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