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KCL-22

  • Specification
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Cat.No.
CSC-C0528
Description
Established from the pleural effusion of a 32-year-old woman with Philadelphia chromosome-positive CML in blast crisis in 1981; described to contain the t(9;22) leading to BCR-ABL1 e13-a2 (b2-a2) fusion gene and a p53 mutation
Species
Human
Source
chronic myeloid leukemia in blast crisis
Recommended Medium
Culture Properties
suspension
Morphology
round, single cells in suspension, sometimes in small clusters
Karyotype
Human hyperdiploid karyotype with 3.3% polyploidy - 51(47-51)<2n>X, -X, +1, +6, +8, +8, +14, +22, del(1)(p22), t(9;22)(q34;q11), add(17)(p12-13), i(21q), der(22)t(9;22)(q34;q11) - carries Phx2 - matches published karyotype
Quality Control
Mycoplasma: contamination was eliminated with Mycoplasma Removal Agent (MRA), then negative in microbiological culture, RNA hybridization, PCR assays
Immunology: CD3 -, CD4 +, CD13 +, CD14 (+), CD15 +, CD19 -, CD25 -, HLA-DR -
Viruses: PCR: EBV -, HBV -,
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 4.5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

For research use only. Not for any other purpose.

  • Q & A
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Q:
What is the FISH profile for leukemia?
A:

A FISH test looks for a small number of specific changes in genes or chromosomes in chronic lymphocytic leukemia (CLL) cells. You may have a range of blood tests to diagnose chronic lymphocytic leukemia (CLL) and throughout treatment. These may include cytogenetic tests.

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Average Rating: 5.0    |    1 Scientist has reviewed this product

Consistency and reliability

As a researcher, I highly recommend this tumor cell product for its consistency and reliability.

24 Nov 2022


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