KCL-22

Cat.No.: CSC-C0528

Species: Human

Source: chronic myeloid leukemia in blast crisis

Morphology: round, single cells in suspension, sometimes in small clusters

Culture Properties: suspension

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Cat.No.

CSC-C0528

Description

Established from the pleural effusion of a 32-year-old woman with Philadelphia chromosome-positive CML in blast crisis in 1981; described to contain the t(9;22) leading to BCR-ABL1 e13-a2 (b2-a2) fusion gene and a p53 mutation

Species

Human

Source

chronic myeloid leukemia in blast crisis

Recommended Medium

90% RPMI-1640 + 10% h.i. FBS

Culture Properties

suspension

Morphology

round, single cells in suspension, sometimes in small clusters

Karyotype

Human hyperdiploid karyotype with 3.3% polyploidy - 51(47-51)<2n>X, -X, +1, +6, +8, +8, +14, +22, del(1)(p22), t(9;22)(q34;q11), add(17)(p12-13), i(21q), der(22)t(9;22)(q34;q11) - carries Phx2 - matches published karyotype

Quality Control

Mycoplasma: contamination was eliminated with Mycoplasma Removal Agent (MRA), then negative in microbiological culture, RNA hybridization, PCR assays
Immunology: CD3 -, CD4 +, CD13 +, CD14 (+), CD15 +, CD19 -, CD25 -, HLA-DR -
Viruses: PCR: EBV -, HBV -,

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 4.5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

What is the FISH profile for leukemia?

A FISH test looks for a small number of specific changes in genes or chromosomes in chronic lymphocytic leukemia (CLL) cells. You may have a range of blood tests to diagnose chronic lymphocytic leukemia (CLL) and throughout treatment. These may include cytogenetic tests.

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Average Rating: 5.0 | 1 Scientist has reviewed this product

Consistency and reliability

As a researcher, I highly recommend this tumor cell product for its consistency and reliability.

24 Nov 2022

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