Established from the transplantable rat ascites hepatoma AH-601 induced by feeding with aminoazotoluene; the hepatoma was passaged by intraperitoneal transplantation though JAR-2 rats
adherent, epithelial-like cells growing in monolayers
Rat hyperdiploid karyotype with 15% polyploidy - 47(45-49)
Mycoplasma: negative in DAPI,microbiological culture, RNA hybridization assays
Viruses: ELISA: reverse transcriptase negative; PCR: SMRV -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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