Normal rat epithelial cells from the small intestine; cells were described to synthesize fibronectin and collagen; no continuous cell line: about 10-15 doublings after thawing of the stock cells
normal small intestine
adherent, transparent, epitheloid cells growing as monolayers (polymorphy of cell forms)
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Viruses: ELISA: reverse transcriptase negative, SMRV -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 1-2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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