Established from the ascitic fluid of a Sprague-Dawley rat (adult female, 240-days-old) with a rat mammary tumor in 1977; tumor was produced by injection of cultured cells from DMBA-induced mammary tumor (DMBA = 9,10-di-methyl-1,2-benzanthracene); derived from identical tumor as HH-16 cl.2/1; cells were described to produce invasive adenocarcinomas in S.D. rats upon s.c. injection with remote metastases in lymph nodes and lung
Rat flat-moded near-tetraploid karyotype with 10% polyploidy - 79-84<4n>
adherent epitheloid cells growing in monolayers
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Viruses: ELISA: reverse transcriptase negative; PCR: SMRV -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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