Description
Established from the ascitic fluid of a Sprague-Dawley rat (adult female, 240-days-old) with a rat mammary tumor in 1977; tumor was produced by injection of cultured cells from DMBA-induced mammary tumor (DMBA = 9,10-di-methyl-1,2-benzanthracene); derived from identical tumor as HH-16 cl.2/1; cells were described to produce invasive adenocarcinomas in S.D. rats upon s.c. injection with remote metastases in lymph nodes and lung
Culture Properties
monolayer
Morphology
adherent epitheloid cells growing in monolayers
Karyotype
Rat flat-moded near-tetraploid karyotype with 10% polyploidy - 79-84<4n>
Quality Control
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Viruses: ELISA: reverse transcriptase negative; PCR: SMRV -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no.
If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
USA: 1-631-626-9181
Europe: 44-208-144-6005
