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Specification
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Cat.No.

CSC-C0199

Description

Established from the peripheral blood of a 30-year-old man with erythroleukemia (AML M6) in relapse (after treatment for Hodgkin lymphoma) in 1980; cells were described to be capable of spontaneous and induced globin synthesis and to carry the JAK2 V617F mutation

Species

Human

Source

erythroleukemia

Recommended Medium

90% RPMI-1640 + 10% h.i. FBS

Culture Properties

suspension

Morphology

round, large to occasionally giant polynucleated, single cells in suspension; few cells adherent

Karyotype

Human hypotriploid karyotype with 2.3% polyploidy - 63(60-64)<3n>XYY, -2, -9, -10, -10, -11, -14, -16, -16, -17, -19, +20, +21, +2mar, del(2)(q32), t(3;6)(p13;q16), der(5)t(5;17)(q10;q10), der(6)t(1;6)(p13;p21), der(7)add(7)(p14;q32), add(8)(p21), der(9)t

Quality Control

Mycoplasma: contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, PCR assays Immunology: CD3 -, CD13 +, CD14 -, CD19 -, CD33 +, CD41a +, CD71 +, CD235a +
Viruses: ELISA: reverse transcriptase negative;

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

CM-1448Y

SuperCult® RPMI 1640 Medium

INQUIRY

CS-001Y

PremSera® FBS

INQUIRY

For research use only. Not for any other purpose.

Q & A
Customer Review
Product Information Sheet

What is the role of circulating tumor cells?

Circulating tumor cells (CTCs) are cells that are shed from the primary tumor and circulate in the blood, and their metastasis and formation of a secondary tumor are closely associated with cancer-related death. Therefore, regulating tumor metastasis through CTCs can be a novel strategy to fight cancer.

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Average Rating: 4.0 | 1 Scientist has reviewed this product

High-quality

The high quality of Creative Bioarray's cell products ensures consistency in my experiments. I'd highly recommend this to anyone in the field.

05 Apr 2023

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