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HD-MAR-2

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Cat.No.
CSC-C0667
Description
Established in 1977 from the pleural effusion of a 20-year-old man with a recurrent T-lymphoblasic Non-Hodgkin lymphoma after treatment of the originally diagnosed Hodgkin lymphoma
Organism
Human
Karyotype
Human flat-moded hypertetraploid karyotype; 92-99<4n>XXYY, -4, +8, +19, +20, +20, +21, +21, +22, +22, del(1)(q21), add(6)(q11)/add(6)(q25), der(9)t(9;10)(p22;?p13)t(9;14)(q35;q11.2)x2, der(14)t(9;14)x2-4, der(17;19)(q10;q10)x2, add(19)(p11), del(19)(q11),
Source
T cell leukemia
Culture Properties
suspension
Morphology
round cells growing singly and in clusters in suspension
Recommended Medium
80-90% RPMI-1640 + 10-20% h.i. FBS
Quality Control
Mycoplasma: negative in microbiological culture, PCR assays
Immunology: CD2 +, CD3 +, CD4 +, CD5 +, CD6 +, CD7 +, CD8 +, CD13 -, CD19 -, CD34 -, TCRalpha/beta +, TCRgamma/delta -
Viruses: PCR: EBV -, HBV -, HCV -, HIV -, HTLV-I/-II -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
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