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EMT6/MTXR

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Cat.No.
CSC-C9500J
Description
The drug-resistant cell line EMT6/MTXR was derived from the mouse mammary tumour cell line EMT6/P by growth in increasing concentrations of methotrexate. EMT6/MTXR did not hyperexpress P-glycoprotein and did not show cross-resistance to other drugs of the multi-drug resistance (MDR) phenotype. It is recommended to culture the cells without drug on resuscitation until first passage or growth has been fully established.
Tissue of Origin
mammary tumour
Organism
Mouse
Culture Properties
Adherent
Morphology
Epithelial
Recommended Medium
MEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1µg/ml methotrexate + 15% Foetal Bovine Serum (FBS). Resuscitate in drug-free medium; add drug once growth is fully established.
Storage
Creative Bioarray ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use.
Never can cryopreserved cells be kept at -20 °C.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
CSC-6256W
CSC-6264W
CSC-6284W
CSC-6285W
CSC-6286W
CSC-6287W
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