The drug-resistant cell line EMT6/MTXR was derived from the mouse mammary tumour cell line EMT6/P by growth in increasing concentrations of methotrexate. EMT6/MTXR did not hyperexpress P-glycoprotein and did not show cross-resistance to other drugs of the multi-drug resistance (MDR) phenotype. It is recommended to culture the cells without drug on resuscitation until first passage or growth has been fully established.
Tissue of Origin
MEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1µg/ml methotrexate + 15% Foetal Bovine Serum (FBS). Resuscitate in drug-free medium; add drug once growth is fully established.
Creative Bioarray ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use.
Never can cryopreserved cells be kept at -20 °C.
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