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EM-3
- Specification
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Cat.No.
CSC-C0291
Description
Established from the bone marrow of a 5-year-old Caucasian girl with Philadelphia chromosome-positive CML (chronic myeloid leukemia) in second relapse in 1980 (after bone marrow transplantation day +47); sister cell line of EM-2
Species
Human
Source
chronic myeloid leukemia in blast crisis
Culture Properties
suspension
Morphology
single, round to oval cells in suspension
Karyotype
Human hypertriploid karyotype - 73(69-77)<3n>X, -X, -X, t(9;22)(q34;q11), i(17q)
Quality Control
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: CD3 -, CD4 -, CD13 +, CD14 -, CD15 +, CD19 -, CD33 +, HLA-DR -
Viruses: ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV -, HTLV-I/II -, S
Immunology: CD3 -, CD4 -, CD13 +, CD14 -, CD15 +, CD19 -, CD33 +, HLA-DR -
Viruses: ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV -, HTLV-I/II -, S
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 5-7 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
- Q & A
- Customer Review
Q:
Why 5% CO2 for cell culture?
A:
5% CO2 is needed to buffer the system to ensure that the normal physiological pH is maintained for optimum cell growth. CO2 will react with water to form carbonate.
Average Rating: 5.0 | 1 Scientist has reviewed this product
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I am happy that the cell product arrived at the expected delivery date.
09 May 2023
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