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EM-2

  • Specification
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Cat.No.
CSC-C0292
Description
Established from the bone marrow of a 5-year-old Caucasian girl with Philadelphia chromosome positive chronic myeloid leukemia (CML) in second relapse in 1980 (after bone marrow transplantation day +28); sister cell line of EM-3
Species
Human
Source
chronic myeloid leukemia in blast crisis
Recommended Medium
Culture Properties
suspension
Morphology
single, round to oval cells in suspension
Karyotype
Human hypertriploid karyotype with hypotetraploid sideline - 74(70-86)<3n>X, -X, -X, +3, +4, +6, +6, +6, +8, -9, +11, -14, -14, +15, +17, -19, +21, +22, +mar - der(5)t(5;?)(q13-15;?), der(9)t(9;22)(q34;q11), i(17q)x2 - in some cells up to three copies of
Quality Control
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: CD3 -, CD4 +, CD13 +, CD14 -, CD15 +, CD19 -, CD33 +, CD34 -, HLA-DR -
Viruses: ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

For research use only. Not for any other purpose.

  • Q & A
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Q:
How does pH affect cell culture?
A:

Changes in pH in the cell culture environment can alter virtually every cellular process like metabolism, cell growth, and membrane potential. Extracellular pH is slightly alkaline and typically 7.3-7.4, while intracellular pH is slightly lower at 7.2.

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Average Rating: 5.0    |    1 Scientist has reviewed this product

Reliable results

The tumor cell product I purchased was of the required purity and produced reliable results in my experiments.

04 May 2023


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