EM-2

Cat.No.: CSC-C0292

Species: Homo sapiens (Human)

Source: Bone Marrow

Morphology: single, round to oval cells in suspension

Culture Properties: suspension

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Cat.No.

CSC-C0292

Description

Established from the bone marrow of a 5-year-old Caucasian girl with Philadelphia chromosome positive chronic myeloid leukemia (CML) in second relapse in 1980 (after bone marrow transplantation day +28); sister cell line of EM-3

Species

Homo sapiens (Human)

Source

Bone Marrow

Recommended Medium

90% RPMI-1640 + 10% h.i. FBS

Culture Properties

suspension

Morphology

single, round to oval cells in suspension

Karyotype

Human hypertriploid karyotype with hypotetraploid sideline - 74(70-86)<3n>X, -X, -X, +3, +4, +6, +6, +6, +8, -9, +11, -14, -14, +15, +17, -19, +21, +22, +mar - der(5)t(5;?)(q13-15;?), der(9)t(9;22)(q34;q11), i(17q)x2 - in some cells up to three copies of

Disease

Chronic Myeloid Leukemia

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: CD3 -, CD4 +, CD13 +, CD14 -, CD15 +, CD19 -, CD33 +, CD34 -, HLA-DR -
Viruses: ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Synonyms

EM2

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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