Established from the pleural effusion of a 28-year-old man with American-type Burkitt lymphoma in 1977
Human hypotetraploid karyotype with 4% polyploidy; 86(83-89)<4n>XXYY, -2, -4, -10, -10, -12, -14, -16, -16, +22, +22, der(5)t(2;5)(p24;q14)x2, del(6)(q15)x2 , t(8;14)(q24;q32)x2, add(14)(q32), der(18)add(18)(q21)x2; carries t(8;14) effecting IGH@-MYC rear
round to polygonal cells, growing singly or in small clusters in suspension
Mycoplasma: contamination was eliminated with Plasmocin, then negative in microbiological culture, PCR assays
Immunology: CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 +, CD138 -, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykap
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 3 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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