Established from the pleural effusion of a 10-year-old boy with Burkitt lymphoma (refractory, terminal) in 1975
Human near diploid karyotype with 6% polyploidy - 46<2n>XY, t(8;14)(q24;q32)
round to polygonal, single or clustered cells in suspension
Mycoplasma: contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: CD3 -, CD10 +, CD13 -, CD19 +, CD34 -, CD37 +, CD38 +, CD80 +, CD138 -, HLA-DR +, c
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at 12 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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