CRO-AP5

Cat.No.: CSC-C0330

Species: Homo sapiens (Human)

Source: Pleural Effusion

Morphology: single round to pleomorph cells growing in suspension

Culture Properties: suspension

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Cat.No.

CSC-C0330

Description

Established in 1998 from the pleural effusion of a 35-year-old HIV-positive homosexual man with primary effusion lymphoma (PEL) and a history of Kaposis sarcoma; cells are HHV-8-positive, but HIV-negative

Species

Homo sapiens (Human)

Source

Pleural Effusion

Recommended Medium

80-85% RPMI-1640 + 15-20% h.i. FBS

Culture Properties

suspension

Morphology

single round to pleomorph cells growing in suspension

Karyotype

Human hyperdiploid karyotype with 12% polyploidy - 44-49<2n>X, -Y, +8, +2mar, del(4)(q25), add(8)(p11), dup(12)(q11q14), del(14)(q24) - sideline with i(8q) - resembles published karyotype

Disease

Primary Effusion Lymphoma

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: CD3 -, CD10 -, CD13 -, CD19 -, CD34 -, CD37 +, cyCD79a -, CD138 -, HLA-DR +, sm/cyIgG -, sm/cyIgM -, sm/cykappa -, cm/cylambda -
Viruses: ELISA: reverse trans

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 3-4 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Synonyms

CRO-AP/5; CROAP5; AP5

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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For research use only. Not for any other purpose.