Description
Established in 1998 from the pleural effusion of a 35-year-old HIV-positive homosexual man with primary effusion lymphoma (PEL) and a history of Kaposis sarcoma; cells are HHV-8-positive, but HIV-negative
Karyotype
Human hyperdiploid karyotype with 12% polyploidy - 44-49<2n>X, -Y, +8, +2mar, del(4)(q25), add(8)(p11), dup(12)(q11q14), del(14)(q24) - sideline with i(8q) - resembles published karyotype
Culture Properties
suspension
Morphology
single round to pleomorph cells growing in suspension
Quality Control
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: CD3 -, CD10 -, CD13 -, CD19 -, CD34 -, CD37 +, cyCD79a -, CD138 -, HLA-DR +, sm/cyIgG -, sm/cyIgM -, sm/cykappa -, cm/cylambda -
Viruses: ELISA: reverse trans
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 3-4 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no.
If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
USA: 1-631-626-9181
Europe: 44-207-097-1828
