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CRO-AP5
- Specification
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Cat.No.
CSC-C0330
Description
Established in 1998 from the pleural effusion of a 35-year-old HIV-positive homosexual man with primary effusion lymphoma (PEL) and a history of Kaposis sarcoma; cells are HHV-8-positive, but HIV-negative
Species
Human
Source
B cell lymphoma
Culture Properties
suspension
Morphology
single round to pleomorph cells growing in suspension
Karyotype
Human hyperdiploid karyotype with 12% polyploidy - 44-49<2n>X, -Y, +8, +2mar, del(4)(q25), add(8)(p11), dup(12)(q11q14), del(14)(q24) - sideline with i(8q) - resembles published karyotype
Quality Control
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: CD3 -, CD10 -, CD13 -, CD19 -, CD34 -, CD37 +, cyCD79a -, CD138 -, HLA-DR +, sm/cyIgG -, sm/cyIgM -, sm/cykappa -, cm/cylambda -
Viruses: ELISA: reverse trans
Immunology: CD3 -, CD10 -, CD13 -, CD19 -, CD34 -, CD37 +, cyCD79a -, CD138 -, HLA-DR +, sm/cyIgG -, sm/cyIgM -, sm/cykappa -, cm/cylambda -
Viruses: ELISA: reverse trans
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 3-4 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
- Q & A
- Customer Review
Q:
Does DAPI stain both live and dead cells?
A:
DAPI can stain dead cells it prefers to stain dead cells. This has to do with the membrane permeability of dead cells. It cannot stain live cells with intact membranes unless used at high concentrations.
Average Rating: 4.0 | 1 Scientist has reviewed this product
High viability
Cell viability is a key aspect of tumor cell product quality, and Creative Bioarray's products have good performance.
16 Jan 2023
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