Established from the malignant primary effusion lymphoma (PEL) of a 42-year-old HIV+ man at diagnosis (prior to therapy) without a previous history of Kaposis sarcoma; cells were shown to carry the human herpesvirus 8 (HHV-8), but not the Epstein-Barr virus (EBV) and not the human immunodeficiency virus (HIV)
Human flat-moded hypotetraploid karyotype - 80-88<4n>XX, -Y, -Y, -1, -2, +6, -7, -10, -13, -13, -22, -22, +6mar, i(1q), add(2)(p2?), add(3)(q2?7), der(4;9)(q10;q10)x1-2, del(5)(p13p15)x1-2, del(5)(q2?1)x1-2, der(6)t(6;7)(q15;q11)add(7)(q3?5), del(8)(p11),
single, round cells growing in suspension
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: CD4 -, CD10 -, CD13 -, CD19 -, CD20 -, CD34 -, CD37 -, cyCD79a -, CD80 -, CD138 -, HLA-DR +, sm/cyIgG -, sm/cyIgM -, sm/cykappa -, sm/cylambda -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 4 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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