Established in 1996 from the diagnostic pre-treatment pleural effusion sampled shortly before death of a 49-year-old HIV+ homosexual man with primary effusion lymphoma (PEL) and previous history of Kaposis sarcoma; autopsy revealed involvement of serous membranes without formation of solid tumor mass; cells were described to be EBV+, HHV-8+ and to carry a BCL6 point mutation
Human hyperdiploid karyotype with 12% polyploidy - 48(47-48)<2n>X, +X, -Y, +12, add(?Y)(p11), add(1)(q43.1), der(15)t(8;15)(q11;q25), der(22)t(1;22)(q21;q13) - sideline with add(9)(q13) - resembles published karyotype
lymphoblast-like cells growing in clumps or as single cells in suspension
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: CD3 -, CD10 -, CD13 -, CD19 -, CD20 -, CD34 -, CD37 (+), cyCD79a -, CD80 -, CD138 +, HLA-DR +, sm/cyIgG -, sm/cyIgM -, sm/cykappa -, sm/cylambda -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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