COR-L23/5010 has been derived from the parent line, COR-L23 by continuous exposure to increasing concentrations of doxorubicin (also known as adriamycin). The cells overexpress multidrug resistance-associated protein (MRP) and express a 190kDa membrane-protein connected to the degree of drug-resistance. COR-L23/5010 have a higher resistance than COR-L23/R.
Aggregates in suspension
RPMI 1640 + 2mM Glutamine + 1.0µg/ml doxorubicin + 10% FBS
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Never can cryopreserved cells be kept at -20 °C.
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