COLO-677

CSC-C2560

Originally described to be derived from the tumor-containing left axillary lymph node of a 39-year-old white man with small lung cell carcinoma (T3 N3 M3) in 1989; however, DNA fingerprinting and cytogenetic analysis showed cross-contamination with cell line RPMI-8226; RPMI-8226 was established from the peripheral blood of a 61-year-old man with multiple myeloma (IgG lambda-type) at diagnosis in 1966

Homo sapiens (Human)

Blood; Peripheral Blood

90% RPMI-1640 + 10% h.i. FBS

monolayer

mostly adherent round cells growing in monolayers

Human highly rearranged hypotriploid karyotype with 17% polyploidy - 62(58-62)<3n>XY, -X/Y, -2, -4, -4, -6, -8, -9, -9, -10, -13, -15, -16, +5mar - add(1)(p21), der(3)t(1;3)(q22;q24), add(4)(q32), add(5)(p11), del(7)(q32), add(11)(p14), add(13) (p11)x2, a

Plasma Cell Myeloma

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin -, cytokeratin-8 -, cytokeratin-18 -, desmin -, endothel -, GFAP -, HMB-45 -, neurofilament -, vimentin +
Viruses: ELISA: reverse transcriptase ne

Frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

COLO 677; COLO #677; COLO677; Colorado 677

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