COLO-677

Cat.No.: CSC-C2560

Species: Homo sapiens (Human)

Source: Blood; Peripheral Blood

Morphology: mostly adherent round cells growing in monolayers

Culture Properties: monolayer

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Cat.No.

CSC-C2560

Description

Originally described to be derived from the tumor-containing left axillary lymph node of a 39-year-old white man with small lung cell carcinoma (T3 N3 M3) in 1989; however, DNA fingerprinting and cytogenetic analysis showed cross-contamination with cell line RPMI-8226; RPMI-8226 was established from the peripheral blood of a 61-year-old man with multiple myeloma (IgG lambda-type) at diagnosis in 1966

Species

Homo sapiens (Human)

Source

Blood; Peripheral Blood

Recommended Medium

90% RPMI-1640 + 10% h.i. FBS

Culture Properties

monolayer

Morphology

mostly adherent round cells growing in monolayers

Karyotype

Human highly rearranged hypotriploid karyotype with 17% polyploidy - 62(58-62)<3n>XY, -X/Y, -2, -4, -4, -6, -8, -9, -9, -10, -13, -15, -16, +5mar - add(1)(p21), der(3)t(1;3)(q22;q24), add(4)(q32), add(5)(p11), del(7)(q32), add(11)(p14), add(13) (p11)x2, a

Disease

Plasma Cell Myeloma

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin -, cytokeratin-8 -, cytokeratin-18 -, desmin -, endothel -, GFAP -, HMB-45 -, neurofilament -, vimentin +
Viruses: ELISA: reverse transcriptase ne

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Synonyms

COLO 677; COLO #677; COLO677; Colorado 677

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

COLO-677 cells were established from ascitic fluid of a patient with metastatic colorectal carcinoma. They exhibit morphology consistent with late passage colorectal cancer cells. These cells have been used primarily as an in vitro tumor model system for studying progression, therapy and chemoresistance mechanisms in colorectal cancer. As established cancer cells from ascites fluid, the cells grow as floating cells or loosely attached cells in culture, and rapidly proliferate in normal culture conditions.

The cells have been found to have a highly aneuploid karyotype. Dysregulated signaling in cell cycle arrest, apoptosis and metabolic changes have also been observed. Studies have utilized this cell line as a tool to understand tumor cell survival, oxidative stress and drug resistance in aggressive CRC. COLO-677 is also commonly used for drug screening or mechanistic studies to discover potential therapeutic targets.

Understanding the Radiobiological Mechanisms Induced by ¹⁷⁷Lu-DOTATATE in Comparison to External Beam Radiation Therapy

¹⁷⁷Lu-DOTATATE radionuclide therapy (RNT) effectively stabilizes neuroendocrine tumours (NETs), yet its radiobiology is largely borrowed from external-beam radiotherapy (EBRT). Delbart et al. compared key radiobiological endpoints and PARP-inhibitor (PARPi) radiosensitisation between ¹⁷⁷Lu-DOTATATE and EBRT in SSTR-expressing cancer cells to determine whether RNT merits dedicated mechanistic studies.

The radiosensitivity of six SSTR-expressing human cancer cell lines to ¹⁷⁷Lu-DOTATATE was previously characterized. Multiple myeloma lines (COLO-677: 33% ± 2%; EJM: 22% ± 2%) and melanoma HBL (26% ± 4%) showed highest sensitivity, with significant survival reduction at day 10. Intermediate sensitivity was observed in melanoma MM162 (13% ± 3%) and GEP MIA-PACA-2 (14% ± 3%), while GEP HT-29 was resistant. External beam radiotherapy (2 Gy) also induced time-dependent cytotoxicity (Fig. 1a), with day 10 survival of 42% (COLO-677), 45% (EJM), 51% (MIA-PACA-2), 61% (HBL), 62% (MM162), and 86% (HT-29)-the latter consistent with its reported radioresistance. EBRT produced significantly greater reduction than ¹⁷⁷Lu-DOTATATE in all lines. Notably, radiosensitivity rankings differed between modalities: HBL was as sensitive as myeloma lines to ¹⁷⁷Lu-DOTATATE but less responsive to EBRT (Fig. 1b).

Effect of 177Lu-DOTATATE and EBRT on the survival of melanoma (HBL and MM162), multiple myeloma (COLO-677 and EJM) and GEP (MIA-PACA-2 and HT-29) cell lines. — Fig. 1. Effect of ¹⁷⁷Lu-DOTATATE and EBRT on the survival of melanoma (HBL and MM162), multiple myeloma (COLO-677 and EJM) and GEP (MIA-PACA-2 and HT-29) cell lines (Delbart W, Karabet J, *et al*., 2022).

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For research use only. Not for any other purpose.