Description
Established from the peripheral blood of a 36-year-old woman with chronic myeloid leukemia (CML) in blast crisis in 1987; cells were described to express T cell surface markers and to carry the BCR-ABL1 translocation e13-a2 (b2-a2) producing the p210 BCR-ABL protein
Karyotype
Human near tetraploid karyotype with diploid sideline - 92<4n>XXX, t(6;7)(q24;q35)x2, del(11)(q22.3)x2
Culture Properties
suspension
Morphology
single, round cells in suspension
Quality Control
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: CD2 -, cyCD3 +, CD3 (+), CD4 +, CD5 +, CD6 +, CD7 +, CD8 +, CD13 -, CD19 -, CD34 -, TCRalpha/beta (+), TCRgamma/delta -
Viruses: ELISA: reverse transcriptase
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no.
If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
USA: 1-631-626-9181
Europe: 44-207-097-1828
