CML-T1

Cat.No.: CSC-C0103

Species: Homo sapiens (Human)

Source: Blood; Peripheral Blood

Morphology: single, round cells in suspension

Culture Properties: suspension

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Cat.No.

CSC-C0103

Description

Established from the peripheral blood of a 36-year-old woman with chronic myeloid leukemia (CML) in blast crisis in 1987; cells were described to express T cell surface markers and to carry the BCR-ABL1 translocation e13-a2 (b2-a2) producing the p210 BCR-ABL protein

Species

Homo sapiens (Human)

Source

Blood; Peripheral Blood

Recommended Medium

90% SuperCult® IMDM or RPMI-1640 + 10% h.i. FBS

Culture Properties

suspension

Morphology

single, round cells in suspension

Karyotype

Human near tetraploid karyotype with diploid sideline - 92<4n>XXX, t(6;7)(q24;q35)x2, del(11)(q22.3)x2

Disease

Chronic Myeloid Leukemia

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: CD2 -, cyCD3 +, CD3 (+), CD4 +, CD5 +, CD6 +, CD7 +, CD8 +, CD13 -, CD19 -, CD34 -, TCRalpha/beta (+), TCRgamma/delta -
Viruses: ELISA: reverse transcriptase

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Synonyms

CML T1; CMLT-1; CMLT1

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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For research use only. Not for any other purpose.