CL-11

Cat.No.: CSC-C0494

Species: Homo sapiens (Human)

Source: Intestine; Colon

Morphology: adherent epitheloid cells growing in monolayers and forming islets

Culture Properties: monolayer

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Cat.No.

CSC-C0494

Description

Established from the primary tumor at the left colon (a moderately differentiated colorectal carcinoma of TNM stage 3); sister cell line of mucoid growing cell line CL-11M; cells were described as expressing tumor-associated carcino-embryonic antigen (CEA)

Species

Homo sapiens (Human)

Source

Intestine; Colon

Recommended Medium

80%DMEM/Ham's F12 + 20% h.i. FBS.

Culture Properties

monolayer

Morphology

adherent epitheloid cells growing in monolayers and forming islets

Karyotype

Human near-diploid karyotype with 12% polyploidy - 46(45-47)<2n>XY, -6, +7, -17, -18, +2-3 mar, +1-4 supernumerary r/dm, add(8)(q24) - sideline with idem, +7

Disease

Colon Adenocarcinoma

Quality Control

Mycoplasma: negative in microbiological culture, PCR assays
Immunology: cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin -
Viruses: PCR:

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Synonyms

CL11

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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