BL-70

Cat.No.: CSC-C0339

Species: Human

Source: Burkitt lymphoma

Morphology: single, round cells growing non-adherent in suspension

Culture Properties: suspension

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Cat.No.

CSC-C0339

Description

Established from the tumor tissue at relapse of a 16-year-old Caucasian boy with Burkitt lymphoma; cells were described to be EBV-negative

Species

Human

Source

Burkitt lymphoma

Recommended Medium

80-90% RPMI-1640 + 10-20% h.i. FBS (possibly add 1 mM sodium pyruvate + 50 μM mercaptoethanol)

Culture Properties

suspension

Morphology

single, round cells growing non-adherent in suspension

Karyotype

Human hyperdiploid karyotype with 4% polyploidy - 47(42-49)<2n>XY, +7, inv(1)(p21q21), del(2)(q33), t(8;14)(q24;q32), t(12;22)(q21.1;q13.2)

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: CD3 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, CD138 -, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa +, sm/cylambda -
Viruses: ELISA: reverse transcri

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

Where does fetal bovine serum come from?

Fetal bovine serum (FBS) is a common component of animal cell culture media. It is harvested from bovine fetuses taken from pregnant cows during slaughter.

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Average Rating: 5.0 | 1 Scientist has reviewed this product

Reliable products

These cell products have shown negative results in several tests including mold, bacteria, and fungus, so we trust Creative Bioarray's products.

13 Jan 2023

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For research use only. Not for any other purpose.