Established from the bone marrow of a Caucasian woman with EBV-negative Burkitt lymphoma with translocation t(8;22) after EBV infection in culture
Human hyperdiploid karyotype; 47(44-48)<2n>XX, +12, del(1)(q23), der(3)t(1;3)(q23;q2?2), t(8;22)(q24;q11); carries t(8:22) effecting rearrangement of IGL@ with MYC; matches unpublished karyotypic data
mostly single round cells in suspension, partly in small clusters
Mycoplasma: contamination was eliminated with Baytril (enrofloxacin), then negative in microbiological culture, PCR assays
Immunology: CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 +, CD138 -, HLA-DR +, sm/cyIgG -, sm/cyIg
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 6 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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