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BE-13
- Specification
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Cat.No.
CSC-C0443
Description
Established from bone marrow cells of an 11-year-old girl with T cell acute lymphoblastic leukemia (T-ALL) in relapse; cell lines BE-13 and PEER show identical DNA fingerprints and hence share common origin, presumably due to cross-contamination; however, their diploid and tetraploid karyotypes indicative of earlier and later passage numbers, respectively, suggest that BE-13 is derived from PEER, rather than vice-versa; carries NUP214-ABL1 fusion gene
Species
Human
Source
bone marrow
Culture Properties
suspension
Morphology
single round cells growing in suspension
Karyotype
Human flat-moded hypotetraploid karyotype - 81-89<4n>XXXX, +1, +4, -9, -9, -10, -16, -21, del(1)(q13.2), add(1)(p11-21), del(4)(q11q31.2)/i(4p)x2, i(4q)del(4q31.2)/i(4q)add(4)(q31)x2, del(5)(q14q21)x2, del(6)(q22)x2, del(9)(p22)x2, der(17)t(17;?21)(p11;q1
Quality Control
Mycoplasma: contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: CD2 -, CD3 +, CD4 +, CD5 +, CD6 +, CD7 +, CD8 -, CD13 -, CD19 -, CD34 -, TCRalpha/beta -
Viru
Immunology: CD2 -, CD3 +, CD4 +, CD5 +, CD6 +, CD7 +, CD8 -, CD13 -, CD19 -, CD34 -, TCRalpha/beta -
Viru
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
- Q & A
- Customer Review
Q:
Why is in situ hybridization important?
A:
The objective of in situ hybridization is to determine the presence or absence of DNA or RNA sequences of interest, as well as to localize these sequences to specific cells or chromosomal sites.
Average Rating: 5.0 | 1 Scientist has reviewed this product
Expected results
We achieved the expected experimental results thanks to the high purity and viability of Creative Bioarray's products.
22 Nov 2022
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