BCBL-1

Cat.No.: CSC-C0665

Species: Homo sapiens (Human)

Source: Ascites

Morphology: round/multiformed cells growing singly in suspension

Culture Properties: suspension

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Cat.No.

CSC-C0665

Description

Established from the peritoneal effusion of a 40-year-old man (HIV+) with B-NHL (primary effusion lymphoma, PEL); cells were shown to carry the human herpesvirus type 8 (HHV-8), but not the Epstein-Barr virus (EBV)

Species

Homo sapiens (Human)

Source

Ascites

Recommended Medium

80% RPMI-1640 + 20% h.i. FBS

Culture Properties

suspension

Morphology

round/multiformed cells growing singly in suspension

Karyotype

Human highly rearranged flat-moded hypertetraploid karyotype with near-diploid and near-octaploid sidelines; 91-100<4n>XX, -Y, -Y, +2, +2, +6, +7, +7, +8, -9, -10, -11, +12, -13, -13, +15, -16, -22, -22, +2, -5mar, dup(1)(q12q44), del(1)(p11)dup(1)(q12q44

Disease

Primary Effusion Lymphoma

Quality Control

Mycoplasma: negative in microbiological culture, PCR assays
Immunology: CD3 -, CD10 -, CD13 -, CD19 -, CD20 -, CD34 -, CD38 +, cyCD79a -, CD80 -, CD138 +, HLA-DR +, sm/cyIgG -, sm/cyIgM -, sm/cykappa -, sm/cylambda -
Viruses: PCR: EBV -, HBV -, HCV -, HHV

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 4 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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For research use only. Not for any other purpose.