Established from the tumor tissue of a 76-year-old woman with metastasizing papillary thyroid carcinoma in 1992
Human near-diploid karyotype with hexaploid sideline - 70/71(69-72)<3n>XX/XXX, +5, -7, -8, +11, +12, -13, +15, +19, -22, +mar - i(1p), 1qh+, t(5;5)(p13;p15), add(10)(p11), add(12)(p11), del(17)(p11), del(17)(p11)dup(17)(q11q23), add(19)(q13) - resembles p
spindle- or round-shaped cells growing adherently as monolayer
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM -, GFAP -, neurofilament -,
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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