380

Cat.No.: CSC-C0215

Species: Homo sapiens (Human)

Source: Blood; Peripheral Blood

Morphology: round, small, single cells in suspension

Culture Properties: suspension

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Cat.No.

CSC-C0215

Description

Established from the peripheral blood of a 15-year-old boy with acute lymphoblastic leukemia (ALL FAB L2) in relapse in 1983; cells were described to be EBNA-negative and to carry t(8;14) and t(14;18) leading to IGH@-BCL2 (IGH-BCL2) and MYC-IGH@ (MYC-IGH) fusion genes, respectively

Species

Homo sapiens (Human)

Source

Blood; Peripheral Blood

Recommended Medium

80-90% RPMI-1640 + 10-20% h.i. FBS

Culture Properties

suspension

Morphology

round, small, single cells in suspension

Karyotype

Human near diploid karyotype with 1.8% polyploidy - 46(43-47)<2n>XY, -14, t(8;14;18)(q24;q32;q21), +der(14)t(8;14;18)(q24;q32;q21) - corresponds to published karyotype

Disease

Childhood B Acute Lymphoblastic Leukemia

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, PCR assays
Immunology: CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, CD80 -, CD138 +, HLA-DR +, sm/cyIgG -, sm/cy IgM -, sm/cykappa -, sm/cylambda -
Viruses: ELISA: reverse transcript

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 10 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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For research use only. Not for any other purpose.