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Cat.No.
CSC-C0215
Description
Established from the peripheral blood of a 15-year-old boy with acute lymphoblastic leukemia (ALL FAB L2) in relapse in 1983; cells were described to be EBNA-negative and to carry t(8;14) and t(14;18) leading to IGH@-BCL2 (IGH-BCL2) and MYC-IGH@ (MYC-IGH) fusion genes, respectively
Species
Human
Source
peripheral blood
Culture Properties
suspension
Morphology
round, small, single cells in suspension
Karyotype
Human near diploid karyotype with 1.8% polyploidy - 46(43-47)<2n>XY, -14, t(8;14;18)(q24;q32;q21), +der(14)t(8;14;18)(q24;q32;q21) - corresponds to published karyotype
Quality Control
Mycoplasma: negative in DAPI, microbiological culture, PCR assays
Immunology: CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, CD80 -, CD138 +, HLA-DR +, sm/cyIgG -, sm/cy IgM -, sm/cykappa -, sm/cylambda -
Viruses: ELISA: reverse transcript
Immunology: CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, CD80 -, CD138 +, HLA-DR +, sm/cyIgG -, sm/cy IgM -, sm/cykappa -, sm/cylambda -
Viruses: ELISA: reverse transcript
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 10 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
- Q & A
- Customer Review
Q:
What is cell viability in the tissue?
A:
Viable tissues are defined as those that are capable of living. Viability assays, therefore, measure attributes of tissue when it is alive. Tissues have multiple attributes in which viability can be assessed.
Average Rating: 5.0 | 1 Scientist has reviewed this product
Easy to culture
Following the instructions provided by Creative Bioarray, these cells are easy to culture and operate in the experiments.
27 Aug 2022
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