380

Cat.No.: CSC-C0215

Species: Human

Source: peripheral blood

Morphology: round, small, single cells in suspension

Culture Properties: suspension

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Cat.No.

CSC-C0215

Description

Established from the peripheral blood of a 15-year-old boy with acute lymphoblastic leukemia (ALL FAB L2) in relapse in 1983; cells were described to be EBNA-negative and to carry t(8;14) and t(14;18) leading to IGH@-BCL2 (IGH-BCL2) and MYC-IGH@ (MYC-IGH) fusion genes, respectively

Species

Human

Source

peripheral blood

Recommended Medium

80-90% RPMI-1640 + 10-20% h.i. FBS

Culture Properties

suspension

Morphology

round, small, single cells in suspension

Karyotype

Human near diploid karyotype with 1.8% polyploidy - 46(43-47)<2n>XY, -14, t(8;14;18)(q24;q32;q21), +der(14)t(8;14;18)(q24;q32;q21) - corresponds to published karyotype

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, PCR assays
Immunology: CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, CD80 -, CD138 +, HLA-DR +, sm/cyIgG -, sm/cy IgM -, sm/cykappa -, sm/cylambda -
Viruses: ELISA: reverse transcript

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 10 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

What is cell viability in the tissue?

Viable tissues are defined as those that are capable of living. Viability assays, therefore, measure attributes of tissue when it is alive. Tissues have multiple attributes in which viability can be assessed.

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Average Rating: 5.0 | 1 Scientist has reviewed this product

Easy to culture

Following the instructions provided by Creative Bioarray, these cells are easy to culture and operate in the experiments.

27 Aug 2022

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For research use only. Not for any other purpose.