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Cat.No.
CSC-C0215
Description
Established from the peripheral blood of a 15-year-old boy with acute lymphoblastic leukemia (ALL FAB L2) in relapse in 1983; cells were described to be EBNA-negative and to carry t(8;14) and t(14;18) leading to IGH@-BCL2 (IGH-BCL2) and MYC-IGH@ (MYC-IGH) fusion genes, respectively
Organism
Human
Karyotype
Human near diploid karyotype with 1.8% polyploidy - 46(43-47)<2n>XY, -14, t(8;14;18)(q24;q32;q21), +der(14)t(8;14;18)(q24;q32;q21) - corresponds to published karyotype
Source
peripheral blood
Culture Properties
suspension
Morphology
round, small, single cells in suspension
Recommended Medium
80-90% RPMI-1640 + 10-20% h.i. FBS
Quality Control
Mycoplasma: negative in DAPI, microbiological culture, PCR assays
Immunology: CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, CD80 -, CD138 +, HLA-DR +, sm/cyIgG -, sm/cy IgM -, sm/cykappa -, sm/cylambda -
Viruses: ELISA: reverse transcript
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 10 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
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