A2058

Viability of A2058 Cancer Cells with Direct and Indirect Ar-CAP Treatments

CAP generates RONS and is a potential cancer therapy tool. Here, Chen's team measured reactive species intensity in plasma gas and RONS concentrations in PBS and cell culture medium. They assessed cell viability after treating cells with plasma in PBS and medium under various conditions and compared the sensitivities of different cells.

A2058 cells were treated with Ar-CAP using two methods: direct exposure (DE) and plasma-treated medium (PTM). For DE, cells were exposed to Ar-CAP in medium volumes of 30, 50, 100, and 150 µL for 0 to 60 seconds (DE0 to DE60) and cultured for 20 hours. For PTM, medium volumes of 30, 50, 100, and 150 µL were treated with plasma for 0 to 60 seconds (P0 to P60) before culturing cells for 20 hours. Cell viability was measured by MTT assay (Fig. 1). In 30 and 150 µL, both DE and PTM decreased cell viability with longer treatment times, but effects were similar. In 50 and 100 µL, DE had stronger lethal effects than PTM. The 150 µL treatments had the most severe lethal effects, likely due to higher plasma intensity generating more RONS and causing stronger oxidative stress.

To investigate short-lived RONS, cells were treated with direct plasma exposure in PBS (DEB) for 0 to 60 seconds or with plasma-treated PBS (PTB) at 30 µL. After 20 minutes, treated PBS was replaced with complete medium and cells were cultured for 20 hours. MTT assays showed that DEB significantly reduced cell viability, with no clear changes over different treatment durations. PTB had minor effects or increased cell viability (Fig. 2a). When 100 µL was used for 50 seconds of plasma exposure, DEB showed a higher lethal effect than PTB (Fig. 2b). These results suggest that short-lived RONS strongly affect cells with DEB treatment.