SW48

Development of A Novel SW48 3D Model

Three-dimensional (3D) cell culture models have emerged as more physiologically relevant alternatives to traditional two-dimensional (2D) models for drug screening and mechanistic studies. This study successfully developed a novel compact spheroid model using the SW48 cell line.

We evaluated the effect of treating the 96-well plates with an anti-adherence solution while culturing SW48 in absence or presence of methylcellulose. In parallel, we cultured two other cell lines, HCT116 and LS174T, as controls. This experiment revealed that the treatment with the anti-adherence solution facilitated the formation of multicellular tumor spheroids (MCTSs) in all three cell lines even in the absence of methylcellulose. We then investigated if the addition of Matrigel or collagen I to the solution treated 96-well plates would lead to the formation of more compact SW48 MCTSs. These conditions allowed the generation of SW48 spheroids, exhibiting a more compact and spherical morphology in the presence of Matrigel and collagen type I compared to cultures in the absence of a matrix or cultured with methylcellulose. We monitored SW48 3D morphology in 7 days culture in anti-adherence-treated 96-well plates revealed that SW48 cells formed smaller and more compact spheroids in Matrigel or collagen I, compared to cultures without hydrogels. While similar in size and compactness, Matrigel-cultured spheroids were notably less round and featured a smoother surface than those in collagen I.