Cloned from the parent line, IPLB-SF-21-AE, derived from pupal ovarian tissue of the fall armyworm (species Spodoptera frugiperda, family Noctuidae, order Lepidoptera); cells are susceptible to infection with MNPV and other Baculoviruses; cells can be used with Baculovirus expression vectors
polymorphic round cells, mostly adherent, a few cells in suspension
80-90% TC 100 medium + 10-20% h.i. FBS (or 80-90% Grace′s insect medium + 10-20% h.i. FBS supplemented with L-Glutamine, hydrolyzed lactalbumin and yeastolate)
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Viruses: PCR: SMRV -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 2-4 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.