Described as secreting a mouse monoclonal antibody (IgG1) reacting with adult rat skeletal muscle type 2A (but not 2B) fibers in immunofluorescence and with undigested type 2A MHCs (myosin heavy chain) as well as both rod and light meromyosin fragments in Western blotting; rat neonatal and embryonic MHCs were not studied by Western blotting; established in 1985 by fusion of NSO myeloma cells with spleen cells from a BALB/c mouse immunized with purified myosin from bovine subcutaneous muscle
single, round cells in suspension
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: secretion of a IgG1 monoclonal antibody
Viruses: ELISA: reverse transcriptase negative; PCR: SMRV -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 3-5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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