These cells were thought to be "established from the malignant urinary bladder carcinoma of a 82-year-old Caucasian woman (stage pTa G1-G2) in 1992"; same donor as for cell line SBC-2; however, DNA fingerprinting and cytogenetic analysis at the Creative Bioarray established unequivocally that this cell line must be considered de facto a subclone of HELA
Human hypertriploid karyotype with 15% polyploidy - 73/74(70-77)<3n>XX/XXX, +1, -4, +5, +6, -9, -9, +11, -13, -15, +16, +16, +17, +17, -22, -22, +4mar, der(1)t(1;14)(p11;q11), i(3q), del(3)(q11), add(5)(p11), i(7q), der(8)t(8;?11)(q24;q11), i(11p)x2, add(
adherent epitheloid cells growing in monolayers
Mycoplasma: contamination was eliminated with Baytril (enrofloxacin), then negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: cytokeratin +, cytokeratin-7 (+), cytokeratin-8 +, cytokeratin-17 +, cytokeratin-18 +, desmin -, end
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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