The transplantable tumor OTT-6050 arose from a 6-day-old embryo grafted into the testis of a strain 129 mouse; this solid subcutaneous tumor was minced in PBS and mice were injected intraperitoneally; ascites containing embryoid bodies developed and cell cultures were initiated from the embryoid bodies
Murine hypotetraploid karyotype - 70(66-72)<4n> - 7-11 centric fusion markers present
adherent epithelial-like cells growing as monolayers
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Viruses: ELISA: reverse transcriptase negative; PCR: SMRV -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 2-6 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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