P3/NSI/1-AG4-1 is a non-secreting clone of P3X63AG8 which is derived from P3K, the induced plasmacytoma MOPC-21; cells do not grow in HAT medium, extensively used in cell fusion studies; cells were described to synthesize kappa chain, but do not secrete free light chain
Hypertriploid murine karyotype with 2% polyploidy - 66(62-72) - 3-4 centric fusion markers present
round cells, single and in small clusters in suspension
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Viruses: ELISA: reverse transcriptase positive; PCR: SMRV -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 3 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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