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P3/NSI/1-AG4-1
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Cat.No.
CSC-C1072
Description
P3/NSI/1-AG4-1 is a non-secreting clone of P3X63AG8 which is derived from P3K, the induced plasmacytoma MOPC-21; cells do not grow in HAT medium, extensively used in cell fusion studies; cells were described to synthesize kappa chain, but do not secrete free light chain
Species
Mouse
Source
myeloma
Recommended Medium
92.5-95% SuperCult® IMDM + 5-7.5% h.i. FBS + 2 mM L-glutamine
Culture Properties
suspension
Morphology
round cells, single and in small clusters in suspension
Karyotype
Hypertriploid murine karyotype with 2% polyploidy - 66(62-72) - 3-4 centric fusion markers present
Quality Control
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Viruses: ELISA: reverse transcriptase positive; PCR: SMRV -
Viruses: ELISA: reverse transcriptase positive; PCR: SMRV -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 3 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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