P3/NSI/1-AG4-1

Cat.No.: CSC-C1072

Species: Mus musculus (Mouse)

Morphology: round cells, single and in small clusters in suspension

Culture Properties: suspension

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Cat.No.
CSC-C1072
Description
P3/NSI/1-AG4-1 is a non-secreting clone of P3X63AG8 which is derived from P3K, the induced plasmacytoma MOPC-21; cells do not grow in HAT medium, extensively used in cell fusion studies; cells were described to synthesize kappa chain, but do not secrete free light chain
Species
Mus musculus (Mouse)
Recommended Medium
92.5-95% SuperCult® IMDM + 5-7.5% h.i. FBS + 2 mM L-glutamine
Culture Properties
suspension
Morphology
round cells, single and in small clusters in suspension
Karyotype
Hypertriploid murine karyotype with 2% polyploidy - 66(62-72) - 3-4 centric fusion markers present
Disease
Mouse Multiple Myeloma
Quality Control
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Viruses: ELISA: reverse transcriptase positive; PCR: SMRV -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 3 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Synonyms
P3/NS1/1-Ag4.1; P3.NS1/1Ag-3.4.1; P3/NSI/1-Ag4-1; P3/NS1/1-Ag4-1; P3/NS1/1-AG4-1; P3/NS1/Ag4-1; P3 NS1 Ag4/1; P3 NS1 Ag4; P3.NS-1/1.Ag4.1; P3-NS/1-Ag4-1; P3-NS1/1-Ag4-1; P3-NS1/1Ag 4.1; P3/NS-1; P3/NS1; NS1/1-Ag4.1; NS1-1 Ag4.1; NS-1-Ag4-1; NS1-Ag4/1; NS1-Ag 4/1; NS1-Ag4; P3-X63-NS/1; P3X63NS1; X63 NS; NS-I/1; NSI/1; NSI/1-Ag4-1; NS-1; NS1; MOPC-21 NS/1; P301; GM03573; GM-3573; GM03573A
Citation Guidance
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