Established from a A/J mouse strain with neuroblastoma C1300; cells were described to be aneuploid and to contain high specific activity of choline acetyltransferase
adherent cells growing in monolayers or small clumps, round cell body with cellular processes
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Viruses: ELISA: reverse transcriptase positive; PCR: SMRV -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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