6-thioguanine resistant mutant of cell line N18 that was established from a mouse of strain A/J with neuroblastoma C1300; cells were described to form cAMP in response to prostaglandin E1 and to be aneuploid (modal chromosome number of 81)
round, single, slightly adherent cells, occasionally with short processes
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Viruses: ELISA: reverse transcriptase positive; PCR: SMRV -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 1-2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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