MFM-223
Cat.No.: CSC-C0461
Species: Homo sapiens (Human)
Source: Pleural Effusion Metastasis
Morphology: adherent cells growing as monolayer with reticulate growth pattern
Culture Properties: monolayer
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Cat.No.
CSC-C0461
Description
Established from the pleural effusion of a post-menopausal breast cancer patient without any prior treatment (tumor: widespread ductal mammary carcinoma T4 N2 M1 with histological grade III); cells were described to express androgen, estrogen and progesterone receptors
Species
Homo sapiens (Human)
Organism
Human
Source
Pleural Effusion Metastasis
Recommended Medium
MEM + 2mM Glutamine + 10% FBS
Culture Properties
monolayer
Morphology
adherent cells growing as monolayer with reticulate growth pattern
Karyotype
Human highly rearranged near-diploid karyotype with 12% polyploidy - 46(45-46)<2n>X, -X, -2, -5, -6, +7, -9, -10, +11, +12, -14, -15, -16, -17, -18, -19, -22, +10mar, add(5)(p15), der(7)?inv(7)(q1?q2?)dup(7)(q31q35), add(7)(q11;hsr), der(7)add(7)(p22)add(
Disease
Breast Carcinoma
Quality Control
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -,
Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -,
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 0.8 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
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