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MFM-223
- Specification
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Cat.No.
CSC-C0461
Description
Established from the pleural effusion of a post-menopausal breast cancer patient without any prior treatment (tumor: widespread ductal mammary carcinoma T4 N2 M1 with histological grade III); cells were described to express androgen, estrogen and progesterone receptors
Species
Human
Organism
Human
Source
breast carcinoma
Recommended Medium
MEM + 2mM Glutamine + 10% FBS
Culture Properties
monolayer
Morphology
adherent cells growing as monolayer with reticulate growth pattern
Karyotype
Human highly rearranged near-diploid karyotype with 12% polyploidy - 46(45-46)<2n>X, -X, -2, -5, -6, +7, -9, -10, +11, +12, -14, -15, -16, -17, -18, -19, -22, +10mar, add(5)(p15), der(7)?inv(7)(q1?q2?)dup(7)(q31q35), add(7)(q11;hsr), der(7)add(7)(p22)add(
Quality Control
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -,
Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -,
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 0.8 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
- Q & A
- Customer Review
Q:
Why do some scholars believe that there is no such thing as black bug contamination?
A:
Firstly, the small black dots do not affect the appressed growth of the cells. Secondly, small black dots appear in culture only after the cells in poor condition have died. Thirdly, it is normal for particles to move in irregular Brownian motion in liquids when their diameter is small enough.
Average Rating: 5.0 | 1 Scientist has reviewed this product
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13 Mar 2021
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