Established from the primary adenosquamous endometrium carcinoma (grading G1-G2) of an 81-year-old woman in 1992; described to form poorly differentiated tumors in nude mice
Human hypodiploid karyotype with 4% polyploidy - 45(43-46)<2n>X, -X, t(13;18)(q14.3-21;q11-12) - resembles published karyotype
epithelial-like adherent cells growing as monolayer
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -,
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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