Established from the primary tumor of a 68-year-old Caucasian woman with moderately differentiated endometrial adenocarcinoma (grade G2) in 1991; cells were described to express androgen receptors (androgen was reported to inhibit cell proliferation), but no estrogen receptors; cells were described to be tumorigenic in nude mice
Human diploid karyotype with 20% polyploidy - 46<2n>XX, no consistent abnormality detected - matches published karyotype
epithelial-like polymorphic cells growing adherently in monolayers
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 -, desmin -, endothel -, EpCAM -, GFAP -, neurofilament -,
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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