Strain: New Zealand White
Production: plasminogen activator; keratin
Note: The cells are positive for keratin by immunoperoxidase staining; this line is extremely sensitive to trypsin. After trypsinization, neutralize the trypsin with culture medium containing 10% serum. Centrifuge the suspension to remove the trypsin, add fresh culture medium and resuspend to set up new cultures
Tests for mycoplasma, bacteria and fungi were negative
Storage and Shipping
Frozen with 52.5% RPMI-1640, 40% FBS, 7.5% DMSO at about 4-5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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