Tumorigenecity: yes, in DBA/2 mice (but only weakly)
Histopathology: lymphoma; chemically induced
Note: this line was derived from the X-ray resistant L5178-R line (KCLB 21722); the cells are sensitive to X-rays, ultraviolet radiation, heat and some potential anti neoplastic drugs (e.g., cis-PAD); the parental cell line was derived from the L5178 thymic lymphoma induced by methylcholanthrene in a DBA/2 mouse; tested and found negative for ectromelia virus (mousepox)
90% Fischer's medium with 0.1g/L sodium pyruvate and 1.125g/L sodium bicarbonate + 10% horse serum
Tests for mycoplasma, bacteria and fungi were negative
Storage and Shipping
Frozen with 52.5% RPMI-1640, 40% FBS, 7.5% DMSO at about 4-5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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