Established from an 8-month-old female DBA/2 mouse with T cell lymphoma in 1985
Murine near diploid karyotype with 13% polyploidy - 39(37-42) - 1 centric fusion marker
grows as single or aggregated round cells in suspension
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Viruses: ELISA: reverse transcriptase positive; PCR: SMRV -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 7 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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