Established from the malignant ascites of a 60-year-old Japanese man with pancreas carcinoma in 1985
epitheloid cells growing adherently in monolayers
Human near-triploid karyotype with 11% polyploidy and 1-2 supernumerary dm - 69/70(66-73)<3n>X, ?del(X)/XY, -Y, -2, -4, +6, +7, +8, -9, -9, +11, +16, +mar - inv(3)(p22q24), i(8q), idic(9)(pter), add(17)(p12) - 2 medium size ring ch present at 10%
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 +, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vim
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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