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HIT-T15

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Cat.No.
CSC-C9429L
Description
Strain: Syrian golden
Production: insulin
Note: This line was established from a primary culture of Syrian hamster islet cells which were transformed with SV40. The cells secrete up to 24ng of insulin in per 10 exp6 cells per 24 hours(passage 60). Insulin in secretion is stimulated by glucose and glucagon, and is suppressed by somatostatin and glucocorticoids. Insulin synthesis decreases with length of time in culture.
Organism
Hamster
Source
pancreas; islet of Langerhans; beta cell
Culture Properties
monolayer
Morphology
epithelial
Recommended Medium
87.5% SuperCult® F-12K Medium with 2mM L-glutamine adjusted to contain 1.5g/L sodium bicarbonate + 10% horse serum + 2.5 % h.i. FBS
Quality Control
Tests for mycoplasma, bacteria and fungi were negative
Storage and Shipping
Frozen with 52.5% RPMI-1640, 40% FBS, 7.5% DMSO at about 4-5 x 10^6 cells/ampoule
Shipping Condition: Room Temperature
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
CSC-6247W
CSC-6248W
CSC-C1081
CSC-C1087
CSC-C1088
CSC-C1105
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