Established from the ascitic fluid of a rat with a tumor which was produced by injecting cultured cells of a chemically-induced (dimethylbenzanthracene) mammary tumor; rat: strain Sprague-Dawley, female, 240 days old, adult; the original tumor was induced by single feeding of DMBA on day 45 after birth; sister cell line of HH-16 cl. 4; cells were described to produce invasive fibrocarcinomas in S.D. rats upon s.c. injection with remote metastases
Rat near diploid karyotype with 11% polyploidy - 39-43<2n>
fibroblastoid growing in a criss-cross pattern
Mycoplasma: negative in microbiological culture, RNA hybridization, PCR assays
Viruses: PCR: SMRV -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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