Established in 2011 from a metastasized group 3 medulloblastoma in the cerebellum of a 3-year-old boy; cells were described to be highly sensitive to histone deacetylase inhibitors
epithelioid cells growing adherently in monolayers
Mycoplasma: negative in PCR assay
Immunology: cytokeratin -, desmin -, endothel -, GFAP -, neurofilament -, vimentin +
Viruses: PCR: EBV -, HBV -, HCV -, HIV -, HTLV-I/-II -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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