Established by fusion of P3-NSI/1-AG4-1 mouse myeloma cells with spleen cells from a mouse immunized with hepatitis B surface antigen; a monoclonal antibody (IgG1) against hepatitis B surface antigen is produced; patented cell line
hybridoma (anti hepatitis B)
single round to oval cells in suspension
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Viruses: ELISA: reverse transcriptase positive
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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