Established from a male bovine fetus at 24-week gestation; vascular endothelium transformed with benzo(a)pyrene in vitro; cell line described as tumorigenic
Hypodiploid bovine karyotype with 15% polyploidy - 58(54-60)<2n> - centric fusion and large submetacentric marker - corresponds to published karyotype
cells grow as adherent multilayer
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Viruses: ELISA: reverse transcriptase negative; PCR: SMRV -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 2.0 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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