EBL

Cat.No.: CSC-C1077

Species: Bovine

Source: embryonic lung cells

Morphology: rectangular-like adherent cells growing as monolayers

Culture Properties: monolayer

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Cat.No.

CSC-C1077

Description

Established from lung of a 7-month-old bovine fetus; useful for detection of Pasteurella multocida toxine

Species

Bovine

Source

embryonic lung cells

Recommended Medium

85% RPMI-1640 or MEM (with Earles salts) + 15% h.i. FBS

Culture Properties

monolayer

Morphology

rectangular-like adherent cells growing as monolayers

Karyotype

Apparently bovine karyotype with bimodal ploidy distribution - 42(40-42)<2n>/80(77-84)<4n> - structurally rearranged with apparent reduction of ch number by centric fusion followed by tetraploidization

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Viruses: ELISA: reverse transcriptase positive; PCR: SMRV -

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 2.5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

How are animal tumor cell lines maintained and propagated in laboratory settings?

Animal tumor cell lines are maintained by regular passaging, ensuring optimal culture conditions, monitoring for contamination, and cryopreserving stocks for long-term storage.

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Average Rating: 5.0 | 1 Scientist has reviewed this product

Bioinformatics resources

To enhance the value and usefulness of the tumor cell product, the supplier could consider providing bioinformatics resources and data analysis tools tailored to analyzing the molecular and genetic data generated from experiments using these cells.

27 Mar 2023

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For research use only. Not for any other purpose.