EBL

Cat.No.: CSC-C1077

Species: Bos taurus (Bovine)

Source: Lung

Morphology: rectangular-like adherent cells growing as monolayers

Culture Properties: monolayer

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Cat.No.

CSC-C1077

Description

Established from lung of a 7-month-old bovine fetus; useful for detection of Pasteurella multocida toxine

Species

Bos taurus (Bovine)

Source

Lung

Recommended Medium

85% RPMI-1640 or MEM (with Earles salts) + 15% h.i. FBS

Culture Properties

monolayer

Morphology

rectangular-like adherent cells growing as monolayers

Karyotype

Apparently bovine karyotype with bimodal ploidy distribution - 42(40-42)<2n>/80(77-84)<4n> - structurally rearranged with apparent reduction of ch number by centric fusion followed by tetraploidization

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Viruses: ELISA: reverse transcriptase positive; PCR: SMRV -

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 2.5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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