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G/G
Cat.No.: CSC-C1383
Species: Mouse
Source: urinary bladder
Morphology: epithelial, adherent cells, growing in up to 3 layers
- Specification
- Q & A
- Customer Review
Cat.No.
CSC-C1383
Description
Established from an adult female C3H/E1 mouse; non-tumorigenic urine bladder cell line immortalized in vitro
Species
Mouse
Source
urinary bladder
Recommended Medium
45% Hams F10 + 45% DMEM + 10% h.i. FBS + 5 μg/ml Human insulin + 5 μg/ml Human transferrin + 50 nM hydrocortisone + 5 ng/ml selenite
Morphology
epithelial, adherent cells, growing in up to 3 layers
Karyotype
Murine near-tetraploid karyotype with 3.6% polyploidy - 80/81(76-83)<4n> - up to 3 (sub)metacentric markers present in most cells arising by centric fusion
Quality Control
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Viruses: ELISA: reverse transcriptase negative; PCR: SMRV -
Viruses: ELISA: reverse transcriptase negative; PCR: SMRV -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 1.5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no.
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