G/G

Specification
Recommended Products

Cat.No.

CSC-C1383

Description

Established from an adult female C3H/E1 mouse; non-tumorigenic urine bladder cell line immortalized in vitro

Species

Mouse

Source

urinary bladder

Recommended Medium

45% Hams F10 + 45% DMEM + 10% h.i. FBS + 5 μg/ml Human insulin + 5 μg/ml Human transferrin + 50 nM hydrocortisone + 5 ng/ml selenite

Morphology

epithelial, adherent cells, growing in up to 3 layers

Karyotype

Murine near-tetraploid karyotype with 3.6% polyploidy - 80/81(76-83)<4n> - up to 3 (sub)metacentric markers present in most cells arising by centric fusion

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Viruses: ELISA: reverse transcriptase negative; PCR: SMRV -

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 1.5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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