Established from an X-irradiated C3H/HeNSIc mouse; cells were described to be stroma-independent, responsive to erythropoietin and to produce hemoglobin (augmented by IL-3)
large, round, single cells in suspension
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Viruses: ELISA: reverse transcriptase positive; PCR: SMRV -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 2-5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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