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EGI-1

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Cat.No.
CSC-C0433
Description
Established from the solid tumor of a 52-year-old Caucasian man with advanced stage malignant bile duct carcinoma (patient had not received any chemotherapy prior to cell explantation) in 1984 and three consecutive passages in female nu/nu mice (NMRI); primary tumor histology: large cell adenocarcinoma of low differentiation, metastasizing (ascites in patient); human character of cell line confirmed by karyotype in passage #34 of in vitro culture (1986)
Organism
Human
Source
bile duct carcinoma
Recommended Medium
90% DMEM + 10% h.i. FBS + 1x MEM amino acids (both essential and non-essential) + 2 mM L-glutamine
Culture Properties
monolayer
Morphology
adherent polymorphic (epitheloid or dendritric) cells growing in monolayers with multilayer foci
Karyotype
Human hypertriploid with 8% polyploidy - 70(68-72)<3n>XYY, -8, -10, +11, -12, -15, +21, +5mar, del(4)(q22), i(5q), i(6p)x2, add(18)(q23)x2, del(21)(q22) - rearrangements of 18q and 21q implicated in gall bladder carcinoma
Quality Control
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vime
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 0.5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
CSC-6321W
CSC-C0316
CSC-C0351
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