DT-40

CSC-C1127

Established in 1985 from a bursal lymphoma, which was induced by infection of a 1 day old chicken with Rous-associated virus type 1 (RAV-1), an avian leukosis retrovirus carrying no cell-derived oncogene; cells are described to retain the ability to rearrange the immunoglobulin light chain gene (IgL) and to exhibit a high frequency of homologous gene targeting

Chicken

B cell line

80% RPMI 1640 medium supplemented with 4.5g/L glucose, 2 mM L-glutamine + 20% h.i. FBS + 50 μM mercaptoethanol

suspension

round cells growing in clumps of 10 to 100 cells in suspension

Mycoplasma: negative in microbiological culture, PCR assays
Viruses: SMRV -

Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

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